GCK Antibody - N-terminal region (OAAB17292)

• Product Number: OAAB17292
• Name: GCK Antibody - N-terminal region (OAAB17292)
• Molecular Weight: 52191 Da
• Isotype: Ig
• NCBI Gene Id: 2645
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: GK, GLK, HK4, HHF3, HKIV, HXKP, LGLK, MODY2, PNDM1, FGQTL3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: GCK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage
• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:50~100
• Immunogen: This GCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCK.
• Additional Information: Tissue Location: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver
• Uniprot ID: P35557
• Protein Name: Glucokinase
• Protein Accession #: NP_000153.1
• Gene Symbol: GCK
• Predicted Species Reactivity: Human, Mouse
• Application: IF
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: HepG2 cell, mouse liver tissue
  
  The anti-GCK Pab (Cat. #OAAB17292) is used in Western blot to detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
• Image 3: HepG2 cell
  
  Confocal immunofluorescent analysis of GCK Antibody (N-term)(Cat#OAAB17292) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).