MUC20 Antibody - C-terminal region (OAAB17284)

• Product Number: OAAB17284
• Name: MUC20 Antibody - C-terminal region (OAAB17284)
• Molecular Weight: 72 kDa
• Isotype: Ig
• NCBI Gene Id: 200958
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.45 mg/ml
• Alias Symbols: MUC-20
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in different individuals. Multiple transcript variants encoding different isoforms have been found for this gene.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.
  Cellular Location: Secreted (Potential). Apical cell membrane. Basolateral cell membrane. Cell projection, microvillus membrane.
  Tissue Location: Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
  FC: 1:10~50
• Immunogen: This MUC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 654-684 amino acids from the C-terminal region of human MUC20.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q8N307
• Protein Name: Mucin-20
• Protein Accession #: NP_001091986.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: MUC20
• Predicted Species Reactivity: Human
• Application: WB, IHC-P, IF, FC
• Image 1: WiDr
  
  MUC20 Antibody (C-term) (Cat. # OAAB17284) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: Nuclear, WiDr
  
  Confocal immunofluorescent analysis of MUC20 Antibody (C-term) (Cat# OAAB17284) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
• Image 3: Human kidney
  
  MUC20 Antibody (C-term) (Cat. # OAAB17284) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MUC20 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.