FYN Antibody - N-terminal region (OAAB17224)

• Product Number: OAAB17224
• Name: FYN Antibody - N-terminal region (OAAB17224)
• Molecular Weight: 60762 Da
• Isotype: Ig
• NCBI Gene Id: 2534
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: SLK, SYN, p59-FYN
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: FYN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta- catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1
• Application Info: WB: 1:1000
  FC: 1:10~50
  IHC-P: 1:50~100
• Immunogen: This FYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human FYN.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Cell membrane. Note=Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking
  Tissue Location: Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes
• Uniprot ID: P06241
• Protein Name: Tyrosine-protein kinase Fyn
• Sample Type Confirmation:

  FYN is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_002028.1
• Gene Symbol: FYN
• Predicted Species Reactivity: Human, Mouse
• Application: WB, FC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Hela
  
  FYN Antibody (N-term) (Cat. # OAAB17224) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: 293
  
  Western blot analysis of FYN (arrow) using rabbit polyclonal FYN Antibody (N-term) (Cat. # OAAB17224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.

  FYN is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Image 3: Mouse Liver
  
  Western blot analysis of anti-FYN (N-term) Pab (Cat. # OAAB17224) in mouse liver tissue lysate. FYN (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.