EphA4 Antibody - N-terminal region (OAAB17129)

• Product Number: OAAB17129
• Name: EphA4 Antibody - N-terminal region (OAAB17129)
• Molecular Weight: 109860 Da
• Isotype: Ig
• NCBI Gene Id: 2043
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: EK8, SEK, HEK8, TYRO1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development Beside its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
  Cellular Location: Cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, axon (By similarity) Cell projection, dendrite (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity) Early endosome (By similarity). Note=Clustered upon activation and targeted to early endosome (By similarity)
  Tissue Location: Ubiquitous.
• Application Info: WB: 1:2000
  WB: 1:1000
  IF: 1:100
  FC: 1:10~50
  IHC-P: 1:50~100
• Immunogen: This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human EphA4.
• Uniprot ID: P54764
• Protein Name: Ephrin type-A receptor 4
• Protein Accession #: NP_004429.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: EPHA4
• Predicted Species Reactivity: Human
• Application: WB, IF, FC, IHC-P
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Chicken, Xenopus
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: CEM, EphA4
  
  Flow cytometric analysis of CEM cells using EphA4 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 3: Human
  
  Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endothelial Lectin(red). Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
• Image 4: 293, EphA4
  
  Western blot analysis of EphA4 (arrow) using EphA4 Antibody (N-term) (Cat.# OAAB17129). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2) (Origene Technologies).
• Image 5: EphA4, Hela
  
  Western blot analysis of anti-EphA4 N-term Pab (Cat. # OAAB17129) in HeLa cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.