MAPK8 Antibody - C-terminal region (OAAB17062)

• Product Number: OAAB17062
• Name: MAPK8 Antibody - C-terminal region (OAAB17062)
• Molecular Weight: 48296 Da
• Isotype: Ig
• NCBI Gene Id: 5599
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: JNK, JNK1, PRKM8, SAPK1, JNK-46, JNK1A2, SAPK1c, JNK21B1/2
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MAPK8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1 In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH
• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:10~50
• Immunogen: This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human MAPK8.
• Additional Information: Cellular Location: Cytoplasm. Nucleus.
• Uniprot ID: P45983
• Protein Name: Mitogen-activated protein kinase 8
• Sample Type Confirmation:

  MAPK8 is supported by BioGPS gene expression data to be expressed in HL60

• Protein Accession #: NP_002741.1
• Gene Symbol: MAPK8
• Predicted Species Reactivity: Human
• Application: WB, IF, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HL-60
  
  Western blot analysis of anti-JNK1 Pab (Cat. # OAAB17062) in HL60 (UV-treated) cell lysate. JNK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

  MAPK8 is supported by BioGPS gene expression data to be expressed in HL60

• Image 2: HepG2, Nuclear
  
  Confocal immunofluorescent analysis of MAPK8 Antibody (C-term) (Cat# OAAB17062) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
• Image 3: Human Breast
  
  MAPK8 Antibody (C-term) (Cat. # OAAB17062) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAPK8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.