MAPK3 Antibody - N-terminal region (OAAB17058)

• Product Number: OAAB17058
• Name: MAPK3 Antibody - N-terminal region (OAAB17058)
• Molecular Weight: 43136 Da
• Isotype: Ig
• NCBI Gene Id: 5595
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MAPK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10) Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This MAPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAPK3.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization
• Uniprot ID: P27361
• Protein Name: Mitogen-activated protein kinase 3
• Sample Type Confirmation:

  MAPK3 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_001035145.1
• Gene Symbol: MAPK3
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Image 1: 293 cell
  
  Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab (Cat. #OAAB17058).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies).
• Image 2: Hela cell
  
  MAPK3 Antibody (N-term) (Cat. #OAAB17058) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 3: human breast carcinoma tissue
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK1 antibody (N-term) (Cat.#OAAB17058), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 4: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.