| Product Number |
OAAB16942 |
| Product Page |
www.avivasysbio.com/pip5k3-antibody-center-region-oaab16942.html |
| Name |
PIP5K3 Antibody - middle region (OAAB16942) |
| Molecular Weight |
237136 Da |
| Isotype |
Ig |
| NCBI Gene Id |
200576 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
CFD, FAB1, HEL37, PIP5K, PIP5K3, ZFYVE29 |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAAB16942 |
| Additional Information |
Precautions: PIP5K3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Additional Information |
Function: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate Required for endocytic-vacuolar pathway and nuclear migration Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes |
| Application Info |
WB: 1:1000
IHC-P: 1:50~100 |
| Immunogen |
This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1561 amino acids from the Central region of human PIP5K3. |
| Additional Information |
Cellular Location: Endosome membrane. Note=Mainly associated with membranes of the late endocytic pathway |
| Uniprot ID |
Q9Y2I7 |
| Protein Name |
1-phosphatidylinositol 3-phosphate 5-kinase |
| Sample Type Confirmation |
PIKFYVE is strongly supported by BioGPS gene expression data to be expressed in HEK293 |
| Protein Accession # |
NP_001171471.1 |
| Gene Symbol |
PIKFYVE |
| Predicted Species Reactivity |
Human |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | breast carcinoma
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
|
| Image 2 | 293
 | Western blot analysis of PIP5K3 (arrow) using rabbit polyclonal PIP5K3 Antibody (Center) (Cat.# OAAB16942). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2) (Origene Technologies). PIKFYVE is strongly supported by BioGPS gene expression data to be expressed in HEK293 |
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