PIM3 Antibody - C-terminal region (OAAB16941)

• Product Number: OAAB16941
• Name: PIM3 Antibody - C-terminal region (OAAB16941)
• Molecular Weight: 35891 Da
• Isotype: Ig
• NCBI Gene Id: 415116
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: pim-3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PIM3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6 Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This PIM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human PIM3.
• Additional Information: Cellular Location: Cytoplasm.
  Tissue Location: Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs
• Uniprot ID: Q86V86
• Protein Name: Serine/threonine-protein kinase pim-3
• Protein Accession #: NP_001001852.2
• Gene Symbol: PIM3
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Mouse Kidney
  
  The anti-PIM3 Pab (Cat. # OAAB16941) is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
• Image 2: Human Spleen
  
  Formalin-fixed and paraffin-embedded human Spleen tissue reacted with PIM3 antibody (C-term) (Cat.# OAAB16941), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: Human hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody (C-term) (Cat.# OAAB16941), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.