PKA C-alpha/PKA C-beta Antibody - N-terminal region (OAAB16744)

• Product Number: OAAB16744
• Name: PKA C-alpha/PKA C-beta Antibody - N-terminal region (OAAB16744)
• Molecular Weight: 40590 Da
• Isotype: Ig
• NCBI Gene Id: 5566
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: CAFD1, PKACA, PPNAD4
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PKA C-alpha/PKA C-beta Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, TRPC1 and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B- alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha- difluoromethylornithine in breast cancer cells when activated RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). TRPC1 activation by phosphorylation promotes Ca(2+) influx, essential for the increase in permeability induced by thrombin in confluent endothelial monolayers. PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Regulates negatively tight junction (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT)
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This PKAC alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-40 amino acids from the N-terminal region of human PRKACA.
• Additional Information: Cellular Location: Cytoplasm. Cell membrane. Nucleus (By similarity). Mitochondrion (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV s
  Tissue Location: Isoform 1 is ubiquitous. Isoform 2 is sperm specific
• Uniprot ID: P17612
• Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Sample Type Confirmation:

  PRKACA is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_002721.1
• Gene Symbol: PRKACA
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Jurkat, Mouse Heart
  
  The anti-PKA/C alpha/beta Pab (Cat. # OAAB16744) is used in Western blot to detect PKA/C alpha in Jurkat cell lysate (lane 1) and mouse heart tissue lysate (lane 2).

  PRKACA is supported by BioGPS gene expression data to be expressed in Jurkat

• Image 2: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.