GPC3 Antibody - N-terminal region (OAAB16662)

• Product Number: OAAB16662
• Name: GPC3 Antibody - N-terminal region (OAAB16662)
• Molecular Weight: 65563
• Isotype: Ig
• NCBI Gene Id: 2719
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 2 mg/ml
• Gene Full Name: glypican 3
• Alias Symbols: DGSX;glypican proteoglycan 3;glypican-3;GTR2-2;heparan sulphate proteoglycan;intestinal protein OCI-5;MXR7;OCI-5;SDYS;secreted glypican-3;SGB;SGBS;SGBS1.
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Description of Target: Glypican-3, glypican-3 isoform 1 precursor [Homo sapiens], glypican-3 isoform 2 precursor [Homo sapiens], glypican-3 isoform 4 precursor [Homo sapiens]
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
  Tissue Location: Highly expressed in lung, liver and kidney.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human Glypican 3 (GPC3).
• Uniprot ID: https://www.uniprot.org/uniprot/P51654
• Protein Name: NP_001158089.1, NP_001158091.1, NP_004475.1, P51654
• Sample Type Confirmation:

  GPC3 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001158089.1,NP_001158091.1,NP_004475.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: GPC3
• Predicted Species Reactivity: Human, Mouse
• Application: Immunohistochemistry-Paraffin, Western blot
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: HepG2
  
  GPC3 Antibody(C35) (cat# OAAB16662) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

  GPC3 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Image 2: Mouse Stomach
  
  GPC3 Antibody(C35) (cat# OAAB16662) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).
• Image 3: Human Liver
  
  Glypican 3 (GPC3) Antibody (N-term) (Cat. # OAAB16662) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Glypican 3 (GPC3) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.