MMP15 Antibody - N-terminal region (OAAB16541)

• Product Number: OAAB16541
• Name: MMP15 Antibody - N-terminal region (OAAB16541)
• Molecular Weight: 75807 Da
• Isotype: Ig
• NCBI Gene Id: 4324
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: MMP-15, MT2MMP, MTMMP2, SMCP-2, MT2-MMP
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MMP15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This MMP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human MMP15.
• Additional Information: Cellular Location: Membrane; Single-pass type I membrane protein; Extracellular side (Potential)
  Tissue Location: Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle
• Uniprot ID: P51511
• Protein Name: Matrix metalloproteinase-15
• Protein Accession #: NP_002419.1
• Gene Symbol: MMP15
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: HL-60, Mouse Brain
  
  The anti-MMP15 N-term Antibody (Cat.# OAAB16541) is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.
• Image 2: Human colon carcinoma
  
  Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: WiDr
  
  Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.