MAGEA12 Antibody - N-terminal region (OAAB16511)

• Product Number: OAAB16511
• Name: MAGEA12 Antibody - N-terminal region (OAAB16511)
• Isotype: Rabbit Ig
• NCBI Gene Id: 4111
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Alias Symbols: CT1.12, MAGE12
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different tissue origins, but not detected in normal tissues, except testis. The function of this gene is unknown. This and other MAGE genes form a gene cluster at chromosome Xq28 region.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines.
  Tissue Location: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  Optimal dilutions should be determined by the end user.
• Immunogen: This MAGEA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human MAGEA12.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P43365
• Protein Name: Melanoma-associated antigen 12
• Protein Accession #: NP_001159858.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification
• Gene Symbol: MAGEA12
• Predicted Species Reactivity: Human
• Application: WB, IHC-P
• Image 1: K562
  
  MAGEA12 Antibody (S96) (Cat. # OAAB16511) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEA12 antibody detected the MAGEA12 protein (arrow).

  MAGEA12 is strongly supported by BioGPS gene expression data to be expressed in K562

• Image 2: Human breast carcinoma
  
  MAGEA12 antibody (N-term) (Cat. # OAAB16511) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.