ACE2 Antibody - N-terminal region (OAAB16460)

• Product Number: OAAB16460
• Name: ACE2 Antibody - N-terminal region (OAAB16460)
• Molecular Weight: 92463 Da
• Isotype: Ig
• NCBI Gene Id: 59272
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ACEH
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Description of Target: ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor for SARS coronavirus.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: ACE2 (SARS Receptor) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This ACE2 (SARS Receptor) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-90 amino acids from the N-terminal region of human ACE2 (SARS Receptor).
• Additional Information: Cellular Location: Processed angiotensin-converting enzyme 2: Secreted
  Tissue Location: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level) Expressed in heart, kidney, testis, and gastrointestinal system
• Uniprot ID: Q9BYF1
• Protein Name: Angiotensin-converting enzyme 2
• Sample Type Confirmation:

  ACE2 is supported by BioGPS gene expression data to be expressed in K562

• Protein Accession #: NP_068576.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: ACE2
• Predicted Species Reactivity: Human
• Application: WB, IHC-P
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: K562
  
  Western blot analysis of anti-ACE2 (SARS Receptor) Antibody (N-term) (Cat.# OAAB16460) in K562 cell line lysates (35ug/lane). ACE2(arrow) was detected using the purified Pab (1:60dilution).

  ACE2 is supported by BioGPS gene expression data to be expressed in K562

• Image 3: Human Testis
  
  Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (N-term) (Cat.# OAAB16460), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.