ULK1 Antibody (Phospho-Ser556) (OAAB16353)

• Product Number: OAAB16353
• Name: ULK1 Antibody (Phospho-Ser556) (OAAB16353)
• Molecular Weight: 112631 Da
• Isotype: Ig
• NCBI Gene Id: 8408
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ATG1, ATG1A, UNC51, hATG1, Unc51.1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: ULK1 (Phospho S556) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation
• Application Info: IF: 1:200
  DB: 1:500
• Immunogen: This ULK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S556 of human ULK1.
• Additional Information: Cellular Location: Cytoplasm, cytosol (By similarity). Preautophagosomal structure (By similarity). Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome (By similarity)
  Tissue Location: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung
• Uniprot ID: O75385
• Protein Name: Serine/threonine-protein kinase ULK1
• Protein Accession #: NP_003556.1
• Gene Symbol: ULK1
• Predicted Species Reactivity: Human
• Application: IF, DB
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of ULK1 Antibody (Phospho S556) Phospho-specific Pab (Cat. # OAAB16353) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
• Image 2: U251
  
  U251