P21CIP1 Antibody (Phospho-Thr57) (OAAB16080)

• Product Number: OAAB16080
• Name: P21CIP1 Antibody (Phospho-Thr57) (OAAB16080)
• Molecular Weight: 18119 Da
• Isotype: Ig
• NCBI Gene Id: 1026
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Phospho-P21CIP1-T57 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex
• Application Info: DB: 1:500
  IHC-P: 1:50~100
• Immunogen: This P21CIP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T57 of human P21CIP1.
• Additional Information: Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Expressed in all adult tissues, with 5-fold lower levels observed in the brain
• Uniprot ID: P38936
• Protein Name: Cyclin-dependent kinase inhibitor 1
• Protein Accession #: NP_000380.1
• Gene Symbol: CDKN1A
• Predicted Species Reactivity: Human
• Application: DB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of anti-Phospho-P21CIP1-T57 Antibody (Cat.# OAAB16080) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.