FOLH1 Antibody - N-terminal region (OAAB10007)

• Product Number: OAAB10007
• Name: FOLH1 Antibody - N-terminal region (OAAB10007)
• Molecular Weight: 84331 Da
• Isotype: Ig
• NCBI Gene Id: 2346
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: PSM, FGCP, FOLH, GCP2, PSMA, mGCP, GCPII, NAALAD1, NAALAdase
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression
  Cellular Location: Cell membrane; Single-pass type II membrane protein
  Tissue Location: Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
• Immunogen: This FOLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of human FOLH1.
• Intended Use: FOLH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q04609
• Protein Name: Glutamate carboxypeptidase 2
• Publications:

  Walder, K. K. et al. Immunohistological and electrophysiological evidence that N-acetylaspartylglutamate is a co-transmitter at the vertebrate neuromuscular junction. Eur. J. Neurosci. 37, 118-29 (2013). 23134476

• Protein Accession #: NP_001014986.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: FOLH1
• Predicted Species Reactivity: Human
• Application: WB, IHC-P, IF
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Pig; Rat
• Image 1: ZR-75-1
  
  FOLH1 Antibody (N-term) (Cat. #OAAB10007) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FOLH1 antibody detected the FOLH1 protein (arrow).
• Image 2: Human prostate carcinoma
  
  FOLH1 Antibody (N-term) (Cat. #OAAB10007)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: ZR-75-1
  
  Confocal immunofluorescent analysis of FOLH1 Antibody (N-term)(Cat#OAAB10007) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).