CDKN1B Antibody - C - terminal region (OAAB09609)

• Product Number: OAAB09609
• Name: CDKN1B Antibody - C - terminal region (OAAB09609)
• Molecular Weight: 22073 Da
• Isotype: Ig
• NCBI Gene Id: 1027
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: KIP1, MEN4, CDKN4, MEN1B, P27KIP1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This CDKN1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the C-terminal region of human CDKN1B.
• Additional Information: Cellular Location: Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity)
  Tissue Location: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney
• Intended Use: CDKN1B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P46527
• Protein Name: Cyclin-dependent kinase inhibitor 1B
• Protein Accession #: NP_004055.1
• Gene Symbol: CDKN1B
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Human Skeletal Muscle
  
  CDKN1B Antibody (C-term) (Cat. #OAAB09609)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDKN1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: Human Uterus
  
  CDKN1B Antibody (C-term) (Cat. #OAAB09609) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the CDKN1B antibody detected the CDKN1B protein (arrow).