APOL1 Antibody (OAAB04367)

Data Sheet
 
Product Number OAAB04367
Product Page www.avivasysbio.com/apol1-antibody-oaab04367.html
Name APOL1 Antibody (OAAB04367)
Molecular Weight 43974 Da
Isotype Rabbit Ig
NCBI Gene Id 8542
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols APOL, APO-L, FSGS4, APOL-I
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-APOL1 antibody - OAAB04367
Application Info WB: 1:1000
IHC-P: 1:10~50
FC: 1:10~50
Other Applications Image 1 Data APOL1-BH3 Domain Antibody (19169) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunogen This APOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-171 amino acids from human APOL1.
Uniprot ID O14791
Protein Name Apolipoprotein L1
Protein Accession # NP_001130012.1
Gene Symbol APOL1
Predicted Species Reactivity Human
Application WB, IHC, FC
Predicted Homology Based on Immunogen Sequence Human
Image 1
MCF-7 cell
APOL1 Antibody (19169) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Image 2
human breast carcinoma
APOL1 Antibody (Cat. #OAAB04367) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APOL1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Image 3
MDA-MB468 cell
Western blot analysis of APOL1 Antibody (Cat. #OAAB04367) in MDA-MB468 cell line lysates (35ug/lane). APOL1(arrow) was detected using the purified Pab.
 

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