| Product Number |
OAAB03788 |
| Product Page |
www.avivasysbio.com/prame-antibody-c-terminal-region-oaab03788.html |
| Name |
PRAME Antibody - C-terminal region (OAAB03788) |
| Molecular Weight |
57890 Da |
| Isotype |
Rabbit Ig |
| NCBI Gene Id |
23532 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
MAPE, OIP4, CT130, OIP-4 |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PRAME antibody - OAAB03788 |
| Application Info |
FC: 1:25
WB: 1:2000
IHC-P: 1:50~100 |
| Other Applications Image 1 Data |
MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
| Immunogen |
This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE. |
| Uniprot ID |
P78395 |
| Protein Name |
Melanoma antigen preferentially expressed in tumors |
| Sample Type Confirmation |
PRAME is strongly supported by BioGPS gene expression data to be expressed in K562 |
| Protein Accession # |
NP_006106.1 |
| Gene Symbol |
PRAME |
| Predicted Species Reactivity |
Human |
| Application |
WB, FC, IHC |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | K562
 | Western blot analysis of MAPE antibody (C-term) in K562 cell lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab. PRAME is strongly supported by BioGPS gene expression data to be expressed in K562 |
| | Image 2 | Human Testis
 | MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
| | Image 3 | K562
 | MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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