CDK6 Antibody - C-terminal region (AVARP03005_T100)

• Product Number: AVARP03005_T100
• Name: CDK6 Antibody - C-terminal region (AVARP03005_T100)
• Protein Size (# AA): 326 amino acids
• Molecular Weight: 37kDa
• NCBI Gene Id: 1021
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Cyclin-dependent kinase 6
• Alias Symbols: MCPH12, PLSTIRE
• Peptide Sequence: Synthetic peptide located within the following region: LLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Mendrzyk,F., (2005) J. Clin. Oncol. 23 (34), 8853-8862
• Description of Target: CDK6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.
• Protein Interactions: PSMA3; DAB1; CDKN2D; CDKN2C; CDKN2B; CCND3; UBC; CCND2; AMY2A; CDKN2A; CDK6; PIGR; DMBT1; WDR33; CDKL3; RB1; CDK4; UCHL1; PML; NUMA1; NFATC3; MYC; MLLT3; MEF2D; MCM2; ISL1; HSP90AA1; HSF1; FOXO3; FOXM1; EZH2; ELK1; DDIT3; ATF6B; CDKN1A; CDC6; CCND1; ZSCAN
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CDK6 (AVARP03005_T100) antibody is Catalog # AAP30155 (Previous Catalog # AAPS08603)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CDK6
• Uniprot ID: Q00534
• Protein Name: Cyclin-dependent kinase 6
• Sample Type Confirmation:

  CDK6 is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_001250
• Purification: Protein A purified
• Nucleotide Accession #: NM_001259
• Tested Species Reactivity: Human
• Gene Symbol: CDK6
• Predicted Species Reactivity: Human, Mouse, Cow, Dog, Horse, Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 84%; Dog: 84%; Horse: 84%; Human: 100%; Mouse: 76%; Pig: 84%; Rabbit: 84%
• Image 1: Human Jurkat
  
  WB Suggested Anti-CDK6 Antibody Titration: 0.625ug/ml
  Positive Control: Jurkat cell lysateCDK6 is supported by BioGPS gene expression data to be expressed in Jurkat
• Image 2: Human Bone Marrow
  
  Rabbit Anti-CDK6 Antibody
  Catalog Number: AVARP03005_T100
  Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
  Observed Staining: Cytoplasm, Nucleus
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec