HK2 Antibody - N-terminal region (ARP54303_P050)

• Product Number: ARP54303_P050
• Name: HK2 Antibody - N-terminal region (ARP54303_P050)
• Protein Size (# AA): 917 amino acids
• Molecular Weight: 102kDa
• NCBI Gene Id: 3099
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Hexokinase 2
• Description:
  View product citations for HK2 Antibody - N-terminal region (ARP54303_P050) on CiteAb
• Alias Symbols: HKII, HXK2
• Peptide Sequence: Synthetic peptide located within the following region: GTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMRGSG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Paudyal,B., (2008) Cancer Sci. 99 (2), 260-266
• Description of Target: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-348 AI278414.1 4-351 349-732 CB160837.1 11-394 c 733-1006 BM912287.1 1-274 c 1007-1333 AI085541.1 1-327 c 1334-1477 AW134604.1 8-151 1478-4058 AF148513.1 1-2581 4059-5498 BC064369.1 2575-4014 5499-5615 BM706373.1 303-419 5616-7109 BC064369.1 4130-5623
• Protein Interactions: UBQLN1; MAGEA12; SUMO2; SUMO3; UBC; EGFR; rev; H2AFV; WDR61; ARIH2; PDLIM1; PYGL; NUBP1; IPO5; HEXB; FNTA; FBXO6; PARK2; UBD; ITGA4; NUDCD3; UBQLN4; SLC2A4; ELAVL1; PTGDS; ZBTB17; PRKCE; IGFBP4;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: 721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.
• Blocking Peptide: For anti-HK2 (ARP54303_P050) antibody is Catalog # AAP54303 (Previous Catalog # AAPP31052)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HK2
• Uniprot ID: P52789
• Protein Name: Hexokinase-2
• Publications:

  Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth. Int J Oncol. 50, 2011-2023 (2017). 28498475

• Sample Type Confirmation:

  HK2 is strongly supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_000180
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000189
• Tested Species Reactivity: Human
• Gene Symbol: HK2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
• Image 1: Human Testis
  
  Rabbit Anti-HK2 Antibody
  Catalog Number: ARP54303_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue
  Observed Staining: Cytoplasm
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 2: Human Skeletal Muscle
  
  Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-HK2 antibody (ARP54303_P050)
• Image 3: Human 721_B
  
  WB Suggested Anti-HK2 Antibody Titration: 1 ug/ml
  Positive Control: 721_B cell lysateHK2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
• Image 4: Human HEP3B
  
  Lanes:
  1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:1000
  Gene Name:
  HK2
  Submitted by:
  Received from annonymous
  
• Image 5: Mouse Testis, Mouse Small Intestine
  
  Host: Rabbit
  Target: HK2
  Positive control (+): Mouse Testis (M-TE)
  Negative control (-): Mouse Small Intestine (M-IN)
  Antibody concentration: 1ug/ml