Product Number |
ARP43300_P050 |
Product Page |
www.avivasysbio.com/rnf25-antibody-middle-region-arp43300-p050.html |
Name |
RNF25 Antibody - middle region (ARP43300_P050) |
Protein Size (# AA) |
459 amino acids |
Molecular Weight |
51kDa |
NCBI Gene Id |
64320 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Ring finger protein 25 |
Alias Symbols |
AO7 |
Peptide Sequence |
Synthetic peptide located within the following region: CREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGG |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Asamitsu,K., (2003) J. Biol. Chem. 278 (29), 26879-26887 |
Description of Target |
RNF25 contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. |
Protein Interactions |
UBE2E3; UBE2D2; UBC; TRIM27; UBE2D1; UBE2U; UBE2D4; UBE2L6; UBE2E2; UBE2E1; UBE2D3; RELA; NKD2; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-RNF25 (ARP43300_P050) antibody |
Additional Information |
IHC Information: Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. |
Blocking Peptide |
For anti-RNF25 (ARP43300_P050) antibody is Catalog # AAP43300 (Previous Catalog # AAPP11375) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human RNF25 |
Uniprot ID |
Q96BH1 |
Protein Name |
E3 ubiquitin-protein ligase RNF25 |
Sample Type Confirmation |
RNF25 is supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_071898 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_022453 |
Tested Species Reactivity |
Human |
Gene Symbol |
RNF25 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100% |
Image 1 | Human HepG2
| WB Suggested Anti-RNF25 Antibody Titration: 0.5 ug/ml Positive Control: HepG2 cell lysateRNF25 is supported by BioGPS gene expression data to be expressed in HepG2 |
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Image 2 | Human Skin
| Human Skin |
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