PDK4 Antibody - N-terminal region (ARP32787_P050)

• Product Number: ARP32787_P050
• Name: PDK4 Antibody - N-terminal region (ARP32787_P050)
• Protein Size (# AA): 411 amino acids
• Molecular Weight: 46kDa
• NCBI Gene Id: 5166
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Pyruvate dehydrogenase kinase, isozyme 4
• Alias Symbols: FLJ40832
• Peptide Sequence: Synthetic peptide located within the following region: MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Description of Target: PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
• Protein Interactions: UBC; DLAT; PDK4; PDHA1; TRIM63;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-PDK4 (ARP32787_P050) antibody is Catalog # AAP32787 (Previous Catalog # AAPP03806)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PDK4
• Uniprot ID: Q16654
• Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
• Protein Accession #: NP_002603
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002612
• Tested Species Reactivity: Human
• Gene Symbol: PDK4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 100%; Rat: 92%
• Image 1: Human 293T
  
  WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml
  Positive Control: Transfected 293T
• Image 2: Human Huh7, HepG2
  
  Sample Type: Huh7 HepG2 (50ug)
  Primary Antibody Dilution: 1:500 
  Image Submitted By: Partha Kasturi
  University of Kansas Medical Center 
• Image 3: Human Muscle
  
  Human Muscle