GLI2 Antibody - middle region (ARP31885_T100)

Data Sheet
 
Product Number ARP31885_T100
Product Page www.avivasysbio.com/gli2-antibody-n-terminal-region-arp31885-t100.html
Name GLI2 Antibody - middle region (ARP31885_T100)
Protein Size (# AA) 1258 amino acids
Molecular Weight 133kDa
NCBI Gene Id 2736
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name GLI family zinc finger 2
Description
Alias Symbols CJS, HPE9, PHS2, THP1, THP2
Peptide Sequence Synthetic peptide located within the following region: RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Ikram,M.S., et al., (2004) J. Invest. Dermatol. 122 (6), 1503-1509
Description of Target GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
Protein Interactions SPOP; SKI; HDAC1; STK36; FBXW11; SUFU; ZIC2; ZIC1; CREB1; TBP;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-GLI2 (ARP31885_T100) antibody
Blocking Peptide For anti-GLI2 (ARP31885_T100) antibody is Catalog # AAP31885 (Previous Catalog # AAPP02680)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
Uniprot ID P10070
Protein Name Zinc finger protein GLI2
Publications

Bosco-Clément, G. et al. Targeting Gli transcription activation by small molecule suppresses tumor growth. Oncogene 33, 2087-97 (2014). 23686308

Coon, V. et al. Molecular therapy targeting Sonic hedgehog and hepatocyte growth factor signaling in a mouse model of medulloblastoma. Mol. Cancer Ther. 9, 2627-36 (2010). 20807782

Ho, L. et al. Gli2 and p53 cooperate to regulate IGFBP-3- mediated chondrocyte apoptosis in the progression from benign to malignant cartilage tumors. Cancer Cell 16, 126-36 (2009). 19647223

Jung, I. H., Jung, D. E., Park, Y. N., Song, S. Y. & Park, S. W. Aberrant Hedgehog ligands induce progressive pancreatic fibrosis by paracrine activation of myofibroblasts and ductular cells in transgenic zebrafish. PLoS One 6, e27941 (2011). 22164219

Kabasawa, Y. et al. Amelogenin positive cells scattered in the interstitial component of odontogenic fibromas. J. Clin. Pathol. 61, 851-5 (2008). 18344235

Kim, J. E. et al. Sonic hedgehog signaling proteins and ATP-binding cassette G2 are aberrantly expressed in diffuse large B-cell lymphoma. Mod. Pathol. 22, 1312-20 (2009). 19593328

Kim, W.-Y. et al. GSK-3 is a master regulator of neural progenitor homeostasis. Nat. Neurosci. 12, 1390-7 (2009). 19801986

Kim, Y. et al. Selective down-regulation of glioma-associated oncogene 2 inhibits the proliferation of hepatocellular carcinoma cells. Cancer Res. 67, 3583-93 (2007). 17440069

Kiprilov, E. N. et al. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. J. Cell Biol. 180, 897-904 (2008). 18332216

Li, Y. et al. Gene expression profiling reveals the heterogeneous transcriptional activity of Oct3/4 and its possible interaction with Gli2 in mouse embryonic stem cells. Genomics 102, 456-67 24121003

Madison, B. B., McKenna, L. B., Dolson, D., Epstein, D. J. & Kaestner, K. H. FoxF1 and FoxL1 link hedgehog signaling and the control of epithelial proliferation in the developing stomach and intestine. J. Biol. Chem. 284, 5936-44 (2009). 19049965

Malek, R., Matta, J., Taylor, N., Perry, M. E. & Mendrysa, S. M. The p53 inhibitor MDM2 facilitates Sonic Hedgehog-mediated tumorigenesis and influences cerebellar foliation. PLoS One 6, e17884 (2011). 21437245

Nakamura, K. et al. Hedgehog promotes neovascularization in pancreatic cancers by regulating Ang-1 and IGF-1 expression in bone-marrow derived pro-angiogenic cells. PLoS One 5, e8824 (2010). 20098680

Non-canonical Hedgehog signaling activation in ovarian borderline tumors and ovarian carcinomas. Int J Oncol. 51, 1869-1877 (2017). 29039491

Omenetti, A. et al. Hedgehog signaling regulates epithelial-mesenchymal transition during biliary fibrosis in rodents and humans. J. Clin. Invest. 118, 3331-42 (2008). 18802480

Omenetti, A. et al. The hedgehog pathway regulates remodelling responses to biliary obstruction in rats. Gut 57, 1275-82 (2008). 18375471

Peacock, Z. S., Cox, D. & Schmidt, B. L. Involvement of PTCH1 mutations in the calcifying epithelial odontogenic tumor. Oral Oncol. 46, 387-92 (2010). 20371205

Regulation of Survivin Isoform Expression by GLI Proteins in Ovarian Cancer. Cells. 8, (2019). 30736319

Schneider, S. et al. Expression of the Sonic hedgehog pathway in squamous cell carcinoma of the skin and the mucosa of the head and neck. Head Neck 33, 244-50 (2011). 20848446

Syn, W.-K. et al. Role for hedgehog pathway in regulating growth and function of invariant NKT cells. Eur. J. Immunol. 39, 1879-92 (2009). 19544307

Takanaga, H. et al. Gli2 is a novel regulator of sox2 expression in telencephalic neuroepithelial cells. Stem Cells 27, 165-74 (2009). 18927476

Tostar, U., Finta, C., Rahman, M. F.-U., Shimokawa, T. & Zaphiropoulos, P. G. Novel mechanism of action on Hedgehog signaling by a suppressor of fused carboxy terminal variant. PLoS One 7, e37761 (2012). 22666390

Wall, D. S. et al. Progenitor cell proliferation in the retina is dependent on Notch-independent Sonic hedgehog/Hes1 activity. J. Cell Biol. 184, 101-12 (2009). 19124651

Yang, L. et al. Hedgehog signaling activation in the development of squamous cell carcinoma and adenocarcinoma of esophagus. Int. J. Biochem. Mol. Biol. 3, 46-57 (2012). 22509480

Youssef, K. K. et al. Identification of the cell lineage at the origin of basal cell carcinoma. Nat. Cell Biol. 12, 299-305 (2010). 20154679

Protein Accession # NP_084655
Purification Protein A purified
Nucleotide Accession # NM_030379
Tested Species Reactivity Human, Mouse
Gene Symbol GLI2
Predicted Species Reactivity Human
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Human: 100%
Image 1
Mouse Gut
Sample Type:
Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop
Primary Antibody Dilution:
1:50
Secondary Antibody:
Goat anti-rabbit-cy3
Secondary Antibody Dilution:
1:1500
Color/Signal Descriptions:
GLI2: Green Neural crest cells:: Red
Gene Name:
GLI2
Submitted by:
Anonymous
Image 2
Jurkat
Host: Rabbit
Target Name: GLI2
Sample Type: Jurkat
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 4.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 6%-18%
Image 3
Human HepG2
Host: Rabbit
Target Name: GLI2
Sample Type: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml
Image 4
Human Intestine
Rabbit Anti-GLI2 Antibody
Catalog Number: ARP31885
Paraffin Embedded Tissue: Human Intestine
Cellular Data: Epithelial cells of intestinal gland
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Image 5
Human Heart
Rabbit Anti-GLI2 Antibody
Catalog Number: ARP31885
Paraffin Embedded Tissue: Human Heart
Cellular Data: Myocardial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Image 6
Human RPMI 8226 Whole Cell
Host: Rabbit
Target Name: GLI2
Sample Tissue: Human RPMI 8226 Whole Cell
Antibody Dilution: 1ug/ml
 

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