| Product Number |
OPPA01638 |
| Product Page |
www.avivasysbio.com/il-27-protein-oppa01638.html |
| Name |
IL 27 Protein (OPPA01638) |
| Protein Size (# AA) |
Recombinant amino acids |
| NCBI Gene Id |
246779 |
| Host |
E. Coli |
| Purity |
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Alias Symbols |
Il, p2, IL-, p28, Il30, IL-27, IL27-A, IL-27-A, IL-27p28 |
| Peptide Sequence |
MFP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHLSDSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLP GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS. |
| Product Format |
Lyophilized from 10mM Sodium Phosphate (NaP) buffer pH-7.5. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Description of Target |
Recombinant Mouse Interleukin-27 |
| Reconstitution and Storage |
Lyophilized Interleukin-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL27 should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OPPA01638 |
| Additional Information |
Solubility: It is recommended to reconstitute the lyophilized Interleukin-27 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| Additional Information |
Biological Activity: Mouse p28 biological activity was measured via dose-dependent inhibition of TGF-beta and IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. |
| Additional Information |
Product Introduction: Interleukin-27 protein is related to IL-12A & is one of the subunits of a heterodimeric cytokine complex. IL-27 interacts with EBV induced gene 3 also called EBI3, and forms a complex that drives rapid expansion of CD4 (+) T cells. IL-27 complex is synergizes with IL-12 in order to trigger the cytokine production of IFN-Gamma of CD4 (+) T cells. The biological effect of IL-27 is mediated by class-I cytokine receptor (WSX1/TCRR). The pro-inflammatory activity of IL-27 is mediated through the growing expression of key molecules involved in the MHC class-I & MHC class-II pathways. Both MHC class-I and MHC-class-II expression are increased in endothelial cells after Interleukin-27 stimulation which suggests that it may play an important role in conferring the immune function on vascular endothelium IL-27p28 subunit can be induced by IFN-beta and during LPS-induced maturation of dendritic cells in type-I IFN-dependent manner through IFN regulatory factor-1 activation. Interleukin-27 regulates Interleukin-12 responsiveness of CD4+ T cells through Stat1-dependent and -independent mechanisms. IL-17 & IL-23 play an important IL-17 role in inflammation. Interleukin-27 possesses potent anti-angiogenic activity that plays an important role in its antitumor and antimetastatic activities. EBV induced gene 3 plays a role, independently from IL-27, in regulating anti-viral or anti-tumoral immune responses. Interleukin-27 is a potent inhibitor of HIV-1 replication in macrophages, CD4+ T cells, peripheral blood mononuclear cells. Interleukin-27 triggers STAT activation and gene transcription.
Product Description: Interleukin-27 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23.7 kDa. The Murine IL-27 is purified by proprietary chromatographic techniques. |
| Uniprot ID |
Q8K3I6 |
| Protein Name |
Interleukin-27 subunit alpha |
| Protein Accession # |
NP_663611.1 |
| Gene Symbol |
IL27 |
| Predicted Species Reactivity |
Mouse |
| Image 1 | |
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