RPA1 Antibody - middle region (ARP56526_P050)

• Product Number: ARP56526_P050
• Name: RPA1 Antibody - middle region (ARP56526_P050)
• Protein Size (# AA): 616 amino acids
• Molecular Weight: 68kDa
• NCBI Gene Id: 6117
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Replication protein A1, 70kDa
• Alias Symbols: HSSB, RF-A, RP-A, REPA1, RPA70, MST075
• Peptide Sequence: Synthetic peptide located within the following region: TLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLSVLSSSTIIANPDIPEA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Tomida,J., (2008) J. Biol. Chem. 283 (14), 9071-9079
• Description of Target: RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. RPA1 binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing
• Protein Interactions: STRA13; UBC; MTUS2; APITD1; PCNA; SUMO2; FBXO18; ATRX; MAP4; CAPRIN1; LMO7; LIG3; LDHA; ITGB1; IMPDH2; ILF3; ILF2; HSPB1; HSPA8; HSPA5; HNRNPU; HNRNPL; HNRNPK; HNRNPH1; HNRNPF; HNRNPD; HNRNPAB; HNRNPA2B1; HNRNPA1; HCFC1; GTF3C2; GTF2I; GTF2H3; GAPDH; XRCC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-RPA1 (ARP56526_P050) antibody is Catalog # AAP56526 (Previous Catalog # AAPP39034)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPA1
• Uniprot ID: P27694
• Protein Name: Replication protein A 70 kDa DNA-binding subunit
• Sample Type Confirmation:

  RPA1 is strongly supported by BioGPS gene expression data to be expressed in 721_B, HeLa

  RPA1 is supported by BioGPS gene expression data to be expressed in MCF7

• Protein Accession #: NP_002936
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002945
• Tested Species Reactivity: Human
• Gene Symbol: RPA1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 93%
• Image 1: Human HepG2
  
  WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: HepG2 cell lysate
• Image 2: Human Hela
  
  Host: Rabbit
  Target Name: RPA1
  Sample Type: Human Hela
  Antibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in HeLa
• Image 3: Human MCF7
  
  Host: Rabbit
  Target Name: RPA1
  Sample Type: Human MCF7
  Antibody Dilution: 1.0ug/mlRPA1 is supported by BioGPS gene expression data to be expressed in MCF7
• Image 4: Human 721_B
  
  Host: Rabbit
  Target Name: RPA1
  Sample Type: Human 721_B
  Antibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells