SIL1 Antibody - N-terminal region (ARP44848_P050)

• Product Number: ARP44848_P050
• Name: SIL1 Antibody - N-terminal region (ARP44848_P050)
• Protein Size (# AA): 461 amino acids
• Molecular Weight: 49kDa
• NCBI Gene Id: 64374
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
• Alias Symbols: BAP, MSS, ULG5
• Peptide Sequence: Synthetic peptide located within the following region: KETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Anttonen,A.K., (2005) Nat. Genet. 37 (12), 1309-1311
• Description of Target: SIL1 is a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in its gene have been associated with Marinesco-Sjogren syndrome.This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.
• Protein Interactions: UBQLN1; FBXO6; UBC; SMAD9; CCND3; HSPA5;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.
• Blocking Peptide: For anti-SIL1 (ARP44848_P050) antibody is Catalog # AAP44848 (Previous Catalog # AAPP25927)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIL1
• Uniprot ID: Q9H173
• Protein Name: Nucleotide exchange factor SIL1
• Protein Accession #: NP_071909
• Purification: Affinity Purified
• Nucleotide Accession #: NM_022464
• Tested Species Reactivity: Human
• Gene Symbol: SIL1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 92%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%
• Image 1: Human HepG2
  
  WB Suggested Anti-SIL1 Antibody Titration: 0.25ug/ml
  Positive Control: HepG2 cell lysate
• Image 2: Human Muscle
  
  Human Muscle
• Image 3: Human Kidney
  
  Rabbit Anti-SIL1 antibody
  Catalog Number: ARP44848
  Formalin Fixed Paraffin Embedded Tissue: Human Kidney
  Primary antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20x
  Exposure Time: 0.5-2.0sec