MECP2 Antibody - N-terminal region (ARP43584_T100)

• Product Number: ARP43584_T100
• Name: MECP2 Antibody - N-terminal region (ARP43584_T100)
• Protein Size (# AA): 486 amino acids
• Molecular Weight: 52kDa
• NCBI Gene Id: 4204
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Methyl CpG binding protein 2 (Rett syndrome)
• Alias Symbols: RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13
• Peptide Sequence: Synthetic peptide located within the following region: MVAGMLGLREEKSEDQDLQGLKEKPLKFKKVKKDKKEDKEGKHEPLQPSA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Robertson,L., (2006) Am. J. Med. Genet. B Neuropsychiatr. Genet. 141 (2), 177-183
• Description of Target: Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
• Protein Interactions: UBC; HECW2; Hipk2; SOX18; WHSC1; HPDL; XPC; CAT; HIST2H3C; HIST1H4F; HIST2H2BE; HIST2H2AC; CBX3; CBX1; SMC3; YBX1; SIN3A; CBX5; ELAVL1; SUMO2; PPARG; YY1; TERF2; SMARCA2; SOX2; SP1; SP3; LOC407840; CDKL5; PRPF40A; SMARCB1; NCOR1; SKI; HMGB1; SPI1; LBR; RB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MECP2 (ARP43584_T100) antibody is Catalog # AAP43584 (Previous Catalog # AAPS13611)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MECP2
• Uniprot ID: P51608
• Protein Name: Methyl-CpG-binding protein 2
• Publications:

  Jungwirth, M., Dear, M. L., Brown, P., Holbrook, K. & Goodchild, R. Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA. Hum. Mol. Genet. 19, 888-900 (2010). 20034025

• Protein Accession #: NP_004983
• Purification: Protein A purified
• Nucleotide Accession #: NM_004992
• Tested Species Reactivity: Human
• Gene Symbol: MECP2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 91%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rat: 93%
• Image 1: Human Jurkat
  
  WB Suggested Anti-MECP2 Antibody Titration: 2.5ug/ml
  Positive Control: Jurkat cell lysate