HNRPA0 Antibody - middle region (ARP40686_T100)

• Product Number: ARP40686_T100
• Name: HNRPA0 Antibody - middle region (ARP40686_T100)
• Protein Size (# AA): 305 amino acids
• Molecular Weight: 34kDa
• NCBI Gene Id: 10949
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Heterogeneous nuclear ribonucleoprotein A0
• Alias Symbols: HNRPA0
• Peptide Sequence: Synthetic peptide located within the following region: KAAVVKFHPIQGHRVEVKKAVPKEDIYSGGGGGGSRSSRGGRGGRGRGGG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Lai,F., (2001) Genomics 71 (2), 235-245
• Description of Target: HNRPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
• Protein Interactions: UBC; SUMO2; SUMO3; STAU1; RPA3; RPA2; RPA1; EED; RNF2; DYNC1LI1; PSMD13; PSMD12; PSMD8; MAP2; DYNC1I2; DHX9; ABCF1; RPS27; TARDBP; CSF2; NPM1; LMNA; AICDA; MAPKAPK2; GADD45A; U2AF2; VCAM1; SRSF1; NKX3-1; MTNR1A; ITGA4; IL7R; HNRNPK; HNRNPH3; HNRNPH2; HNRN
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-HNRNPA0 (ARP40686_T100) antibody is Catalog # AAP40686 (Previous Catalog # AAPY00938)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HNRPA0
• Uniprot ID: Q13151
• Protein Name: Heterogeneous nuclear ribonucleoprotein A0
• Protein Accession #: NP_006796
• Purification: Protein A purified
• Nucleotide Accession #: NM_006805
• Tested Species Reactivity: Human
• Gene Symbol: HNRNPA0
• Predicted Species Reactivity: Human, Cow, Dog, Pig
• Application: IF, IP, WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Human: 100%; Pig: 93%
• Image 1: Human Jurkat
  
  WB Suggested Anti-HNRPA0 Antibody Titration: 0.625ug/ml
  ELISA Titer: 1:312500
  Positive Control: Jurkat cell lysate
• Image 2: Human Heart
  
  Rabbit Anti-HNRPA0 Antibody
  Catalog Number: ARP40686
  Paraffin Embedded Tissue: Human Heart
  Cellular Data: Myocardial cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: Human Liver
  
  Rabbit Anti-HNRPA0 Antibody
  Catalog Number: ARP40686
  Paraffin Embedded Tissue: Human Liver
  Cellular Data: Hepatocytes
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 4: Human Heart
  
  Rabbit Anti-HNRNPA0 Antibody
  Catalog Number: ARP40686
  Paraffin Embedded Tissue: Human cardiac cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 5: HeLa S3, MCF7, K562
  
  Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate
  Primary Antibody Dilution:
  1:4000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  HNRPA0
  Submitted by:
  Anonymous
• Image 6: MCF-7
  
  Sample Type:
  MCF7 cells
  Primary Antibody Dilution:
  1:200
  Secondary Antibody:
  Anti-rabbit-FITC
  Secondary Antibody Dilution:
  1:500
  Color/Signal Descriptions:
  DAPI: Blue hn-RNPA0: Green
  Gene Name:
  HNRPA0
  Submitted by:
  Anonymous
• Image 7: K562
  
  Amount and Sample Type:
  Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPA0 ppt. k562 sample
  IP Antibody:
  HNRPA0
  Amount of IP Antibody:
  
  Primary Antibody:
  HNRPA0
  Primary Antibody Dilution:
  1:4000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  HNRPA0
  Submitted by:
  Anonymous