| Product Number |
ARP40583_P050 |
| Product Page |
www.avivasysbio.com/ncl-antibody-n-terminal-region-arp40583-p050.html |
| Name |
NCL Antibody - N-terminal region (ARP40583_P050) |
| Protein Size (# AA) |
710 amino acids |
| Molecular Weight |
78kDa |
| NCBI Gene Id |
4691 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Nucleolin |
| Alias Symbols |
C23, Nsr1 |
| Peptide Sequence |
Synthetic peptide located within the following region: GKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDED |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Huang,Y., (2006) Blood 107 (9), 3564-3571 |
| Description of Target |
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. |
| Protein Interactions |
HUWE1; PIK3R1; PA2G4; NCL; HNRNPD; AURKA; MDM2; NFE4; CDKN1A; ADAP1; STAU1; ADAP2; UBC; NUP62; ZBTB1; ARFGEF1; RPA3; RPA2; RPA1; ERG; RNF2; SUZ12; EED; EZH2; BMI1; NPM3; EIF3G; XRCC5; PLOD2; NCBP1; MCM2; LMNA; PRMT1; HNRNPK; KCTD12; DNM1; ACLY; SRPK3; TAR |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NCL (ARP40583_P050) antibody |
| Additional Information |
IHC Information: Paraffin embedded skin tissue, tested with an antibody dilution of 5 ug/ml. |
| Blocking Peptide |
For anti-NCL (ARP40583_P050) antibody is Catalog # AAP40583 (Previous Catalog # AAPP22759) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human NCL |
| Uniprot ID |
P19338 |
| Protein Name |
Nucleolin |
| Sample Type Confirmation |
NCL is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_005372 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_005381 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
NCL |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Yeast, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 87%; Dog: 93%; Horse: 93%; Human: 100%; Mouse: 91%; Pig: 86%; Rabbit: 77%; Rat: 93%; Yeast: 83%; Zebrafish: 85% |
| Image 1 | Human Kidney
 | Rabbit Anti-NCL Antibody
Catalog Number: ARP40583
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 2 | Human Lung
 | Rabbit Anti-NCL Antibody
Catalog Number: ARP40583
Paraffin Embedded Tissue: Human Lung
Cellular Data: Alveolar cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human Skin
 | Human Skin |
|
| Image 4 | Human Jurkat
 | WB Suggested Anti-NCL Antibody Titration: 0.2-1 ug/mlPositive Control: JurkatNCL is supported by BioGPS gene expression data to be expressed in Jurkat |
|
