ILF3 Antibody - N-terminal region (ARP40523_P050)

• Product Number: ARP40523_P050
• Name: ILF3 Antibody - N-terminal region (ARP40523_P050)
• Protein Size (# AA): 702 amino acids
• Molecular Weight: 76kDa
• NCBI Gene Id: 3609
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Interleukin enhancer binding factor 3, 90kDa
• Alias Symbols: CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NF90c, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, NFAR-2, NFAR90, TCP110, MPP4110, NF90ctv, NFAR110, MPHOSPH4, NF-AT-90
• Peptide Sequence: Synthetic peptide located within the following region: ALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Nie,Y., (2005) Mol. Cell. Biol. 25 (16), 6956-6963
• Description of Target: ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.
• Protein Interactions: PLSCR1; UBC; FBXW11; RBPMS; HUWE1; MDM2; AURKA; SUMO2; SUMO3; STAU1; IVNS1ABP; LGR4; ERG; RPA3; RPA2; RPA1; EZH2; HNRNPL; SUZ12; RNF2; BMI1; EED; VHL; VEGFA; rev; FBXO6; TARDBP; CSF2; EIF2AK2; ICAM1; IGSF8; SNORD116-22; PABPC1; RNU2-4P; RNU5B-1; RNU5A-1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ILF3 (ARP40523_P050) antibody is Catalog # AAP40523 (Previous Catalog # AAPP22290)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ILF3
• Uniprot ID: Q12906-2
• Protein Name: Interleukin enhancer-binding factor 3
• Sample Type Confirmation:

  ILF3 is strongly supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_004507
• Purification: Affinity Purified
• Nucleotide Accession #: NM_004516
• Tested Species Reactivity: Human
• Gene Symbol: ILF3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 86%; Guinea Pig: 79%; Horse: 93%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 79%
• Image 1: Human HEK293T
  
  WB Suggested Anti-ILF3 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: 293T cell lysateILF3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
• Image 2: Human Heart
  
  Rabbit Anti-ILF3 antibody
  Catalog Number: ARP40523
  Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: Human Liver
  
  Rabbit Anti-ILF3 Antibody
  Catalog Number: ARP40523
  Paraffin Embedded Tissue: Human Liver
  Cellular Data: Hepatocytes
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 4: Human Heart
  
  Rabbit Anti-ILF3 Antibody
  Catalog Number: ARP40523
  Paraffin Embedded Tissue: Human Heart
  Cellular Data: Myocardial cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X