TRIM32 Antibody - N-terminal region (ARP38965_T100)

• Product Number: ARP38965_T100
• Name: TRIM32 Antibody - N-terminal region (ARP38965_T100)
• Protein Size (# AA): 653 amino acids
• Molecular Weight: 72kDa
• NCBI Gene Id: 22954
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Tripartite motif containing 32
• Alias Symbols: HT2A, BBS11, TATIP, LGMD2H, LGMDR8
• Peptide Sequence: Synthetic peptide located within the following region: MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chiang,A.P., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (16), 6287-6292
• Description of Target: TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
• Protein Interactions: PTPN11; PDE9A; PTCD2; CLIP4; KCTD9; UBQLN1; TRIM32; RABAC1; ABI2; SCGB1A1; UBE2E1; UBE2D1; UBC; TP53; SDCBP; UBE2U; SYT6; MYCN; CEP250; CUL3; GPRASP2; GLIS2; YWHAB; TP73; UBE2D3; UBE2D2; HSP90AA1; SFN; YWHAQ; YWHAZ; YWHAH; YWHAG; YWHAE; NOTCH1; UBD; TOP1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TRIM32 (ARP38965_T100) antibody is Catalog # AAP38965 (Previous Catalog # AAPY00774)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM32
• Uniprot ID: Q13049
• Protein Name: E3 ubiquitin-protein ligase TRIM32
• Publications:

  Kudryashova, E., Wu, J., Havton, L. A. & Spencer, M. J. Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Hum. Mol. Genet. 18, 1353-67 (2009). 19155210

  Streich, F. C., Ronchi, V. P., Connick, J. P. & Haas, A. L. Tripartite motif ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism. J. Biol. Chem. 288, 8209-21 (2013). 23408431

• Protein Accession #: NP_036342
• Purification: Protein A purified
• Nucleotide Accession #: NM_012210
• Tested Species Reactivity: Human
• Gene Symbol: TRIM32
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 93%; Mouse: 93%; Rabbit: 93%; Rat: 93%
• Image 1: Human Jurkat
  
  WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/ml
  ELISA Titer: 1:62500
  Positive Control: Jurkat cell lysate