Product Number |
ARP34728_T100 |
Product Page |
www.avivasysbio.com/keap1-antibody-c-terminal-region-arp34728-t100.html |
Name |
KEAP1 Antibody - C-terminal region (ARP34728_T100) |
Protein Size (# AA) |
624 amino acids |
Molecular Weight |
70kDa |
NCBI Gene Id |
9817 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Kelch-like ECH-associated protein 1 |
Alias Symbols |
INrf2, KLHL19 |
Peptide Sequence |
Synthetic peptide located within the following region: TWTFVAPMKHRRSALGITVHQGRIYVLGGYDGHTFLDSVECYDPDTDTWS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Padmanabhan,B., (2006) Mol. Cell 21 (5), 689-700 |
Description of Target |
KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
Protein Interactions |
OXR1; ZSCAN32; LSM3; KLHL3; FBXW11; MAPKBP1; NUDT4; DPP3; RBX1; ATG5; SQSTM1; PTMA; PRKAG1; NFE2L2; MAD2L1; GYPA; ETF1; BCL2; CUL3; RECK; UBC; PGAM5; FAM117B; WDR83; CCNB1IP1; HSP90AA1; XPO1; MCMBP; KEAP1; SLK; MCM3; IKBKB; AMER1; GPRASP2; PRKCE; NFE2L1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-KEAP1 (ARP34728_T100) antibody |
Blocking Peptide |
For anti-KEAP1 (ARP34728_T100) antibody is Catalog # AAP34728 (Previous Catalog # AAPP23660) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human KEAP1 |
Uniprot ID |
Q14145 |
Protein Name |
Kelch-like ECH-associated protein 1 |
Publications |
Protein levels in Keap1-Nrf2 system in human failing heart. Int. J. Cardiol. 225, 62-64 (2016). 27710805 |
Sample Type Confirmation |
KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_987096 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_203500 |
Tested Species Reactivity |
Human |
Gene Symbol |
KEAP1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
Image 1 | Human kidney
| Human kidney |
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Image 2 | HepG2
| Host: Rabbit Target Name: KEAP1 Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1.25ug/mL Peptide Concentration: 1.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
Image 3 | Human HepG2
| WB Suggested Anti-KEAP1 Antibody Titration: 1.0ug/ml Positive Control: HepG2 cell lysateKEAP1 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
Image 4 | Human HCT116 Whole Cell
| Host: Rabbit Target Name: KEAP1 Sample Tissue: Human HCT116 Whole Cell Antibody Dilution: 1ug/ml |
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