TOP2B Antibody - middle region (ARP33079_P050)

• Product Number: ARP33079_P050
• Name: TOP2B Antibody - middle region (ARP33079_P050)
• Protein Size (# AA): 1621 amino acids
• Molecular Weight: 183kDa
• NCBI Gene Id: 7155
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Topoisomerase (DNA) II beta 180kDa
• Alias Symbols: TOPIIB, top2beta
• Peptide Sequence: Synthetic peptide located within the following region: FGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: McNamara,S., (2008) Mol. Cell. Biol. 28 (6), 2066-2077
• Description of Target: TOP2B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: BAZ1B; SUMO2; SUMO3; UBC; EIF6; RPA3; RPA2; RPA1; ERG; WHSC1; NOTCH1; ESR1; NCSTN; SNRNP200; RBM14; HNRNPR; HDAC3; XPC; TOP2A; SNRPE; SMARCE1; SMARCD2; SMARCC2; SMARCC1; SMARCB1; SMARCA4; SMARCA2; SFPQ; S100A9; MSH2; IK; SEPT7; SUMO1; CUL5; NEDD8; RANBP2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TOP2B (ARP33079_P050) antibody is Catalog # AAP33079 (Previous Catalog # AAPP04112)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TOP2B
• Uniprot ID: Q02880-2
• Protein Name: DNA topoisomerase 2-beta
• Protein Accession #: NP_001059
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001068
• Tested Species Reactivity: Human
• Gene Symbol: TOP2B
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human Stomach
  
  WB Suggested Anti-TOP2B Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Human Stomach