| Product Number |
ARP32403_T100 |
| Product Page |
www.avivasysbio.com/lef1-antibody-c-terminal-region-arp32403-t100.html |
| Name |
LEF1 Antibody - C-terminal region (ARP32403_T100) |
| Protein Size (# AA) |
399 amino acids |
| Molecular Weight |
44kDa |
| NCBI Gene Id |
51176 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Lymphoid enhancer-binding factor 1 |
| Alias Symbols |
LEF-1, TCF10, TCF7L3, TCF1ALPHA |
| Peptide Sequence |
Synthetic peptide located within the following region: VKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQ |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Liu,X., et al., (2004) J. Invest. Dermatol. 123 (2), 264-274 |
| Description of Target |
Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1. |
| Protein Interactions |
RNF138; UBC; RNF14; NLK; NARF; STAT5A; PITX2; CTNNB1; CDX1; DPYSL2; nrarp; FHIT; MITF; HINT1; TLE2; TLE1; SETD8; MYB; E2F1; HDAC1; ZBTB3; DACT1; DAZAP2; ALYREF; PIAS4; ALX4; SMAD3; SUMO2; EP300; SMAD1; SMAD2; SMAD4; KPNA1; KPNA2; UBTF; RUNX2; NOTCH1; RUVB |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-LEF1 (ARP32403_T100) antibody |
| Blocking Peptide |
For anti-LEF1 (ARP32403_T100) antibody is Catalog # AAP32403 (Previous Catalog # AAPP03394) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human LEF1 |
| Uniprot ID |
Q9UJU2 |
| Protein Name |
Lymphoid enhancer-binding factor 1 |
| Sample Type Confirmation |
LEF1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_057353 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_016269 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
LEF1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-LEF1 Antibody Titration: 2.0ug/ml
Positive Control: Jurkat cell lysateLEF1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
|
| Image 2 | Human Ovary, Human liver
 | Host: Rabbit
Target: LEF1
Positive control (+): Human Ovary (OV)
Negative control (-): Human liver (LI)
Antibody concentration: 3ug/ml |
|
| Image 3 | Human Intestine
 | Human Intestine |
|
