| Product Number |
ARP32221_T100 |
| Product Page |
www.avivasysbio.com/e2f6-antibody-middle-region-arp32221-t100.html |
| Name |
E2F6 Antibody - middle region (ARP32221_T100) |
| Protein Size (# AA) |
129 amino acids |
| Molecular Weight |
14kDa |
| NCBI Gene Id |
1876 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
E2F transcription factor 6 |
| Alias Symbols |
E2F-6 |
| Peptide Sequence |
Synthetic peptide located within the following region: HEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Kimura,K., et al., (2006) Genome Res. 16 (1), 55-65 |
| Description of Target |
E2F6 is a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants encoding several different isoforms have been found for this gene. |
| Protein Interactions |
TFDP2; TFDP1; KRTAP10-7; LYN; ARAF; APP; PCGF6; RYBP; YAF2; RNF2; SMARCAD1; UBC; L3MBTL2; EZH2; Epc1; EED; KMT2D; KMT2A; RING1; E2F1; BMI1; ZMYND8; ZMYND11; KDM5C; EZH1; CBX3; E2F6; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-E2F6 (ARP32221_T100) antibody |
| Blocking Peptide |
For anti-E2F6 (ARP32221_T100) antibody is Catalog # AAP32221 |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human E2F6 |
| Uniprot ID |
Q6Q9Z5 |
| Protein Name |
E2F6 splice variant f EMBL AAT02641.1 |
| Sample Type Confirmation |
E2F6 is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_997705 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_212540 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
E2F6 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 100%; Guinea Pig: 85%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100% |
| Image 1 | Human HepG2
 | WB Suggested Anti-E2F6 Antibody
Titration: 1.25 ug/ml
Positive Control: HepG2 Whole CellE2F6 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
| Image 2 | Human Lung
 | Rabbit Anti-E2F6 antibody
Catalog Number: ARP32221_T100
Paraffin Embedded Tissue: Human Lung cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
