Product Number |
ARP31531_P050 |
Product Page |
www.avivasysbio.com/eap30-antibody-n-terminal-region-arp31531-p050.html |
Name |
EAP30 Antibody - N-terminal region (ARP31531_P050) |
Protein Size (# AA) |
258 amino acids |
Molecular Weight |
29kDa |
NCBI Gene Id |
11267 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) |
Alias Symbols |
Dot3, EAP30, VPS22 |
Peptide Sequence |
Synthetic peptide located within the following region: MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEF |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Schmidt,A.E., et al., (1999) Biol. Chem. 274 (31), 21981-21985 |
Description of Target |
ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase II interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. EAP30 is a subunit of the ELL complex. EAP30 can interact with ELL and derepress ELL's inhibitory activity in vitro.SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]. |
Protein Interactions |
GOLGA2; VPS25; TRIM54; VAC14; UBC; BCAT1; SUV39H2; ACBD3; DUS3L; WDR12; PRMT6; RPUSD2; KDM1A; EFTUD2; TTC1; HARS; GTF2F1; GTF2E1; ELL; RBP1; MCM2; ADORA1; METTL14; NUDCD3; RILP; VPS36; CHMP6; VPS28; SNF8; TSG101; VPS20; NIF3L1; DVL2; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-SNF8 (ARP31531_P050) antibody |
Additional Information |
IHC Information: Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) IHC Information: Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) |
Blocking Peptide |
For anti-SNF8 (ARP31531_P050) antibody is Catalog # AAP31531 (Previous Catalog # AAPS26305) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human EAP30 |
Uniprot ID |
Q96H20 |
Protein Name |
Vacuolar-sorting protein SNF8 |
Sample Type Confirmation |
SNF8 is supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_009172 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_007241 |
Tested Species Reactivity |
Human |
Gene Symbol |
SNF8 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
Image 1 | Human heart
| Anti-SNF8 / EAP30 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. EAP30 Antibody ARP31531_P050 concentration 5 ug/ml. |
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Image 2 | Human spleen
| Anti-SNF8 / EAP30 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. EAP30 Antibody ARP31531_P050 concentration 5 ug/ml. |
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Image 3 | Human Stomach
| Rabbit Anti-EAP30 Antibody Catalog Number: ARP31531 Paraffin Embedded Tissue: Human Stomach Cellular Data: Epithelial cells of Fundic Gland and Surface Mucous Cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
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Image 4 | Human HepG2
| WB Suggested Anti-EAP30 Antibody Titration: 5.0ug/ml Positive Control: HepG2 cell lysateSNF8 is supported by BioGPS gene expression data to be expressed in HepG2 |
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