| Product Number |
ARP31175_P050 |
| Product Page |
www.avivasysbio.com/e2f4-antibody-c-terminal-region-arp31175-p050.html |
| Name |
E2F4 Antibody - C-terminal region (ARP31175_P050) |
| Protein Size (# AA) |
413 amino acids |
| Molecular Weight |
44kDa |
| NCBI Gene Id |
1874 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
E2F transcription factor 4, p107/p130-binding |
| Alias Symbols |
E2F-4 |
| Peptide Sequence |
Synthetic peptide located within the following region: SSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
DuPree,E.L., et al., (2004) Cancer Res. 64 (13), 4390-4393 |
| Description of Target |
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. |
| Protein Interactions |
RBL2; TFDP1; RBL1; RB1; XPO1; PCM1; HDAC1; POLR1D; CHMP2A; AP3S2; TRIM28; LIN9; ARID3A; NCOR2; HDAC3; AR; CEBPA; LIN54; LIN37; UBC; ATAD2; SIN3A; ASH2L; HCFC1; TRRAP; KAT2A; ID2; E2F1; KMT2A; ID3; SMAD3; NOLC1; TFDP2; NDN; SMAD2; BRCA1; RYBP; RNF144A; FHL |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-E2F4 (ARP31175_P050) antibody |
| Blocking Peptide |
For anti-E2F4 (ARP31175_P050) antibody is Catalog # AAP31175 (Previous Catalog # AAPP01918) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human E2F4 |
| Uniprot ID |
Q16254 |
| Protein Name |
Transcription factor E2F4 |
| Protein Accession # |
NP_001941 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001950 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
E2F4 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Stomach
 | Human Stomach |
|
| Image 2 | Human Intestine
 | Human Intestine |
|
| Image 3 | Human HepG2
 | WB Suggested Anti-E2F4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate |
|
