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Catalog No: OKCA02157
Size:96 Wells
Price: $754.00
SKU
OKCA02157
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)Neat8884-94
Serum (n=4)1:29490-98
Serum (n=4)1:49285-96
Serum (n=4)1:89188-95
ELISA Kit PrincipleAviva Systems Biology ZYX ELISA Kit (Human) (OKCA02157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZYX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZYX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ZYX captured in well.
ELISA Kit Range23.5 pg/mL-1,500 pg/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)9389-97
EDTA plasma (n=4)8884-94
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-ZYX Microplate96 Wells (12 x 8 Well strips)
ZYX Lyophilized Standard 2 x 1.5 ng
100X Biotinylated ZYX Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25XWash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity5.8 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolZYX
Alias SymbolsESP-2, HED-2
NCBI Gene Id7791
Protein NameZyxin
Description of TargetAdhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
Uniprot IDQ15942
Protein Accession #NP_001010972
Nucleotide Accession #NM_001010972
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