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Catalog No: OKCD04391
Size:96WELLS
Price: $700.00
SKU
OKCD04391
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ZPI ELISA Kit (Mouse) (OKCD04391)

Datasheets/ManualsPrintable datasheet for ZPI ELISA Kit (Mouse) (OKCD04391)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
sodium citrate plasma(n=5)85-97%80-99%80-94%89-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serpin A10 (SERPINA10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serpin A10 (SERPINA10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serpin A10 (SERPINA10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serpin A10 (SERPINA10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Sodium Citrate Plasma (n=5)99-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serpin A10 (SERPINA10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serpin A10 (SERPINA10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Zpi Microplate96 Wells (12 x 8 Well strips)
Zpi Lyophilized Standard2
100X Zpi HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma.
Sensitivity0.28 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSerpina10
Gene Full Nameserine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Alias SymbolsP;protein Z-dependent protease inhibitor;PZ-dependent protease inhibitor;PZI;serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10;Serpin A10;ZPI.
NCBI Gene Id217847
Protein NameProtein Z-dependent protease inhibitor
Description of TargetInhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors (By similarity).
Uniprot IDQ8R121
Protein Accession #NP_001288333.1
Nucleotide Accession #NM_001301404.1
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