ZPBP Antibody (OAGA00377)

Catalog No: OAGA00377

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:A431
B:HeLa S3
12% SDS PAGE
OAGA00377 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded SCC25 xenograft, using ZPBP(OAGA00377) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: ZPBP is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida (McLeskey et al., 1998 [PubMed 9378618]).[supplied by OMIM]
  Positive Controls: 293T, A431, H1299, HeLaS3
  Cellular Localization: Secreted
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 29 and 275 (Q9BS86) of ZPBP
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.48 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ZPBP
• Gene Full Name: Zona pellucida-binding protein 1
• Alias Symbols: ZPBP1
• NCBI Gene Id: 11055
• Protein Name: Zona pellucida-binding protein 1
• Uniprot ID: Q9BS86
• Protein Accession #: NP_008940.2
• Molecular Weight: 40 kDa