Catalog No: OKCD00875 (Formerly GWB-KBBUU3)
Size:96WELLS
Price: $725.00
SKU
OKCD00875
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for ZP2 ELISA Kit (Rat) (OKCD00875) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.125 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). No significant cross-reactivity or interference between Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Zp2 |
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Gene Full Name | zona pellucida glycoprotein 2 |
Alias Symbols | zona pellucida 2 glycoprotein;Zona pellucida glycoprotein 2;zona pellucida glycoprotein 2 (sperm receptor);zona pellucida glycoprotein ZP2;zona pellucida protein A;zona pellucida sperm-binding protein 2;Zp-2. |
NCBI Gene Id | 81828 |
Protein Name | Zona pellucida sperm-binding protein 2 |
Description of Target | The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. |
Uniprot ID | O54767 |
Protein Accession # | NP_112412 |
Nucleotide Accession # | NM_031150.1 |
Protein Size (# AA) | 695 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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