ZNRD1 ELISA Kit (Human) (OKCD01041)

Catalog No: OKCD01041 (Formerly GWB-KBBSK9)

Size:96WELLS

Human ZNRD1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zinc Ribbon Domain Containing Protein 1 (ZNRD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zinc Ribbon Domain Containing Protein 1 (ZNRD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zinc Ribbon Domain Containing Protein 1 (ZNRD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Zinc Ribbon Domain Containing Protein 1 (ZNRD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Ribbon Domain Containing Protein 1 (ZNRD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Ribbon Domain Containing Protein 1 (ZNRD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ZNRD1 Microplate: 96 Wells (12 x 8 Well Strips)
  • ZNRD1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ZNRD1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.107 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Zinc Ribbon Domain Containing Protein 1 (ZNRD1). No significant cross-reactivity or interference between Zinc Ribbon Domain Containing Protein 1 (ZNRD1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: POLR1H
• Gene Full Name: RNA polymerase I subunit H
• Alias Symbols: A12.2;DNA-directed RNA polymerase I subunit H;DNA-directed RNA polymerase I subunit RPA12;HTEX6;HTEX-6;hZR14;RNA polymerase I small specific subunit Rpa12;Rpa12;tctex-6;TCTEX6;TEX6;transcription-associated zinc ribbon protein;zinc ribbon domain containing 1;Zinc ribbon domain-containing protein 1;ZNRD1;ZR14.
• NCBI Gene Id: 30834
• Protein Name: DNA-directed RNA polymerase I subunit RPA12
• Description of Target: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors.
• Uniprot ID: Q9P1U0
• Protein Accession #: NP_001265714
• Nucleotide Accession #: NM_001278785.1
• Protein Size (# AA): 126