ZG16B Chemi-Luminescent ELISA Kit (Human) (OKCD04066)

Catalog No: OKCD04066

Size:96WELLS

Human ZG16B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-90%	95-105%	87-105%	86-103%
  EDTA Plasma (n=5)	97-105%	97-104%	88-95%	78-94%
  Heparin Plasma (n=5)	90-97%	89-96%	94-105%	94-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Zymogen Granule Protein 16 Homolog B (ZG16B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zymogen Granule Protein 16 Homolog B (ZG16B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zymogen Granule Protein 16 Homolog B (ZG16B) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-97	86
  EDTA Plasma (n=5)	95-102	98
  Heparin Plasma (n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zymogen Granule Protein 16 Homolog B (ZG16B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zymogen Granule Protein 16 Homolog B (ZG16B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ZG16B Microplate: 96 Wells (12 x 8 Well strips)
  • ZG16B Lyophilized Standard: 2
  • 100X ZG16B Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ZG16B
• Gene Full Name: zymogen granule protein 16B
• Alias Symbols: EECP;endocrine and exocrine protein;HRPE773;jacalin-like lectin domain containing 2;JCLN2;pancreatic adenocarcinoma upregulated factor;PAUF;PRO1567;zymogen granule protein 16 homolog B.
• NCBI Gene Id: 124220
• Protein Name: Zymogen granule protein 16 homolog B
• Description of Target: Zymogen granule protein 16 homolog B
• Uniprot ID: Q96DA0
• Protein Accession #: NP_660295
• Nucleotide Accession #: NM_145252