ZFAT Antibody (OAGA06947)

Catalog No: OAGA06947

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
5% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ZFAT antibody at 1:500 dilution.

ZFAT antibody detects ZFAT protein at cytoplasm and nucleus in mouse placenta by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
ZFAT antibody diluted at 1:500.

ZFAT antibody detects ZFAT protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZFAT protein stained by ZFAT antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ZFAT. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.71 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ZFAT
• Gene Full Name: zinc finger and AT-hook domain containing
• Alias Symbols: AITD3, ZFAT1, ZNF406
• NCBI Gene Id: 57623
• Protein Name: zinc finger protein ZFAT
• Description of Target: This gene encodes a protein that likely binds DNA and functions as a transcriptional regulator involved in apoptosis and cell survival. This gene resides in a susceptibility locus for autoimmune thyroid disease (AITD) on chromosome 8q24. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Uniprot ID: Q9P243
• Protein Accession #: NP_001025110.2
• Nucleotide Accession #: NM_001029939.3