ZFAND6 ELISA Kit (Human) (OKCD09377)

Catalog No: OKCD09377

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ZFAND6 Microplate: 96 Wells (12 x 8 Well strips)
  • ZFAND6 Lyophilized Standard: 2
  • 100X ZFAND6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: ZFAND6
• Gene Full Name: zinc finger AN1-type containing 6
• Alias Symbols: AN1-type zinc finger protein 6;Associated with PRK1 protein;AWP1;protein associated with PRK1;ZA20D3;ZFAND5B;Zinc finger A20 domain-containing protein 3;zinc finger, A20 domain containing 3;zinc finger, AN1-type domain 6.
• NCBI Gene Id: 54469
• Protein Name: AN1-type zinc finger protein 6
• Description of Target: Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes (By similarity).
• Uniprot ID: Q6FIF0
• Protein Accession #: NP_001229840.1
• Nucleotide Accession #: NM_001242911.1