ZEB2 ELISA Kit (Human) : 96 Wells (OKEH02951)

Catalog No: OKEH02951

Size:96T

Human ZEB2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ZEB2 ELISA Kit (Human) (OKEH02951) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ZEB2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZEB2 and incubated. The ZEB2 found in the sample or standards competes with the biotinylated ZEB2 for limited binding sites on the immobilized anti-human ZEB2 antibody. Excess unbound biotinylated ZEB2 and sample or standard ZEB2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZEB2 captured in the well and inversely proportional to the amount of ZEB2 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.9% (n = 20)
  Mean Inter-assay CV%: < 8.8% (n = 20)
• ELISA Kit Component:
  • Anti-ZEB2 Microplate: 96 Wells (12 x 8 Well strips)
  • ZEB2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X ZEB2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.17 ng/mL
• Specificity: Natural and recombinant Human Zinc finger E-box-binding homeobox 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ZEB2
• Gene Full Name: zinc finger E-box binding homeobox 2
• Alias Symbols: HSPC082;SIP1;SIP-1;SMAD interacting protein 1;Smad-interacting protein 1;SMADIP1;ZFHX1B;zinc finger E-box-binding homeobox 2;zinc finger homeobox 1b;Zinc finger homeobox protein 1b.
• NCBI Gene Id: 9839
• Protein Name: O60315
• Description of Target: Zinc finger E-box-binding homeobox 2
• Uniprot ID: https://www.uniprot.org/uniprot/O60315
• Protein Accession #: NP_001165124.1
• Nucleotide Accession #: NM_001171653.1
• Protein Size (# AA): 1214
• Molecular Weight: 136 kDa