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Catalog No: OKCD04065
Size:96WELLS
Price: $850.00
SKU
OKCD04065
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ZEB2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD04065)

Datasheets/ManualsPrintable datasheet for ZEB2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD04065)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zinc Finger Homeobox Protein 1B (ZFHX1B) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Zeb2 Microplate96 Wells (12 x 8 Well strips)
Zeb2 Lyophilized Standard2
100X Zeb2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolZeb2
Gene Full Namezinc finger E-box binding homeobox 2
Alias Symbols9130203F04Rik;D130016B08Rik;S;SIP1;smad-interacting protein 1;Zfhx1b;Zfx1b;Zfxh1b;zinc finger E-box-binding homeobox 2;zinc finger homeobox protein 1b.
NCBI Gene Id24136
Protein NameZinc finger E-box-binding homeobox 2
Description of TargetTranscriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
Uniprot IDQ9R0G7
Protein Accession #NP_001276450
Nucleotide Accession #NM_001289521
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