ZEB2 Antibody - C-terminal region (OABB01108)

Catalog No: OABB01108

Size:100UG

Anti-Smad Interacting Protein 1 antibody, OABB01108, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for ZEB2 is approximately 0.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: ZEB2(Zinc finger E-box-binding homeobox2), also known as SIP1or ZINC FINGER HOMEOBOX 1B(ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation(NURD) complex. By radiation hybrid analysis, Nagase et al.(1998) mapped the ZEB2 gene to chromosome 2. Wakamatsu et al.(2001) mapped the ZEB2 gene to chromosome 2q22. Vandewalle et al.(2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression of SNAI1 in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al.(2008) showed that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Zinc finger E-box-binding homeobox 2(ZEB2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: ZEB2
• Gene Full Name: zinc finger E-box binding homeobox 2
• Alias Symbols: HSPC082;SIP1;SIP-1;SMAD interacting protein 1;Smad-interacting protein 1;SMADIP1;ZFHX1B;zinc finger E-box-binding homeobox 2;zinc finger homeobox 1b;Zinc finger homeobox protein 1b.
• NCBI Gene Id: 9839
• Protein Name: Zinc finger E-box-binding homeobox 2
• Description of Target: Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
• Uniprot ID: O60315
• Molecular Weight: 136447 MW