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Catalog No: OCOA02789
Size:20UG
Price: $275.00
SKU
OCOA02789
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ZEB1 Protein Lysate (OCOA02789)

Datasheets/ManualsPrintable datasheet for ZEB1 Protein Lysate (OCOA02789)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionZEB1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolZEB1
Gene Full Namezinc finger E-box binding homeobox 1
Alias SymbolsAREB6;BZP;delta-crystallin enhancer binding factor 1;DELTAEF1;FECD6;negative regulator of IL2;NIL2A;NIL-2-A zinc finger protein;posterior polymorphous corneal dystrophy 3;PPCD3;TCF8;Transcription factor 8;transcription factor 8 (represses interleukin 2 expression);ZFHEP;ZFHX1A;zinc finger E-box-binding homeobox 1;zinc finger homeodomain enhancer-binding protein.
NCBI Gene Id6935
Protein NameB2RBI8, NM_030751, NP_110378, P37275
Description of TargetcDNA, FLJ95535, highly similar to Homo sapiens transcription factor 8 (represses interleukin 2 expression) (TCF8), mRNA, Homo sapiens zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 2, mRNA, Zinc finger E-box-binding homeobox 1, zinc finger E-box-binding homeobox 1 isoform b [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P37275
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_110378
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_030751
Molecular Weight124.5 kDa
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